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In addition, both of these methods (DTNB and OPT) are highly dependent on the sample conditions, making them relatively less accurate than the HPLC method
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Separation was performed on a Kinetex 2.6 μm C18 100 Å LC column (50 × 2.1 mm) at 40 °C using a flow rate of 0.6 ml min −1
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